October 16, 2010[nb 1] is the 16th day in the month of October 2010.
16:01 EDT: The Team get word that many heroes have fallen to alien invaders.
16:21 EDT: The Team wakes up from their training simulation.
↑Series producer Greg Weisman stated that even though the series does not have a "canon year", the production team based the days of the week on the 2010 calendar. (Weisman, Greg (2011-11-18). Question #13695. Ask Greg. Retrieved 2011-11-18.).